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KMID : 0364820110470010074
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Korean Journal of Microbiology
2011 Volume.47 No. 1 p.74 ~ p.80
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Characteristics of Bacteriocin Produced by Lactococcus lactis ET45 Isolated from Kimchi
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Jeong Seong-Yeop
Park Chan-Sun
Choi Nack-Shick
Yang Hee-Jong
Kim Cha-Young
Yoon Byoung-Dae
Kang Dae-Ook
Ryu Yeon-Woo
Kim Min-Soo
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Abstract
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Bacteriocin-producing lactic acid bacterium having antagonistic activity against Bacillus cereus, was isolated from Kimchi. The selected strain was identified as Lactococcus lactis by the Bergey’s manual and 16S rDNA analysis, and named as L. lactis ET45. The bacteriocin was stable in the pH range 3.0-11.0. The bacteriocin was active over a wide temperature range from 40°C to 121°C. Optimal culture condition for producing bacteriocin was obtained by growing the cells on MRS medium at pH 7.5 and 30°C for 18 h. Antibacterial activity of the bacteriocin was completely disappeared by
proteinase K, and this means that bacteriocin is a proteinous substance. The molecular weight of bacteriocin was estimated to be about 4.5 kDa by tricine sodium dodecyl sulfate polyacryamide gel electrophoresis (TSDS-PAGE).
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KEYWORD
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Bacillus cereus, Lactococcus lactis, bacteriocin, tricine-SDS-PAGE
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